GStorm GS4822
Streptococcus pneumoniae is a leading cause of human diseases such as pneumonia, bacteremia, meningitis, and otitis media. Pneumolysin (Ply) is an important virulence factor of S. pneumoniae and a promising future vaccine target. However, the expansion of clones carrying ply alleles with reduced hemolytic activity has been observed in serotypes associated with outbreaks of invasive disease and includes an allele identified in a highly virulent serotype 1 isolate (ply4496). The virulence of Ply-deficient and ply allelic-replacement derivatives of S. pneumoniae D39 was compared with that of wild-type D39. In addition, the protective immunogenicity of Ply against pneumococci with low versus high hemolytic activity was also investigated. Replacement of D39 ply with ply4496 resulted in a small but statistically significant reduction of virulence. However, both native Ply- and Ply4496-expressing strains were significantly more virulent than a Ply-deficient mutant. While the numbers of both Ply- and Ply4496-expressing isolate cells were higher in the blood than the numbers of Ply-deficient mutant cells, the growth of the Ply4496-expressing strain was superior to that of the wild type in the first 15 h postchallenge. Ply immunization provided protection regardless of the hemolytic activity of the challenge strain. In summary, we show that low-hemolytic-activity Ply alleles contribute to systemic virulence and may provide a survival advantage in the blood. Moreover, pneumococci expressing such alleles remain vulnerable to Ply-based vaccines.

“PCRs were performed using a G-STORM GS482 thermal cycler (Gene Technologies).”
Published ahead of print 25 July 2011, doi: 10.1128/IAI.05418-11 Infect. Immun. October 2011 vol. 79 no. 10 4122-4130 

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Hidden Y Chromosome Mosaicism in 48 Egyptian Patients with Turner’s Syndrome

  • Background. The presence of Y chromosome material in Turner’s syndrome (TS) patients is a risk factor for the development of gonadoblastoma. Although conventional cytogenetic analysis is the definitive diagnosis of TS, low level Y chromosome mosaicism may be missed. Molecular analysis has demonstrated a higher proportion of mosaicism, but there is controversy regarding the prevalence of Y chromosome-derived material in those patients. Aim and Methods. This study was conducted to investigate the prevalence of hidden Y chromosome mosaicism in 48 TS Egyptian patients using polymerase chain reaction (PCR) for molecular DNA analysis of SRY gene and compare our results with those in the literature. Results. None of TS patients had a cytogenetically obvious Y chromosome; Y chromosome material was detected only at molecular analysis. SRY gene was found in 9 TS patients (18.75%) with the classical 45,X karyotype, whereas all other patients were SRY negative. Conclusion. Cytogenetically undetected Y chromosome mosaicism is common in TS patients; these data reinforce the need for adequate diagnosis of Y chromosome material.
  • “The amplification was carried out with a DNA thermal cycler (G-STORM GS482, By Gene Technologies, UK), 33 cycles for (SRY 1F/SRY 2R: 94∘C, 10 min., 94∘C, 45 seconds, 58∘C, 1min., and 72∘C, 2 min.). in those patients. Molecular screening for Y chromosome-derived DNA should be routinely carried out in all TS patients.”
  • http://www.google.co.uk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=182&cad=rja&uact=8&ved=0CCUQFjABOLQB&url=http%3A%2F%2Fdownloads.hindawi.com%2Fjournals%2Fgri%2F2013%2F463529.pdf&ei=2WahU8KEMc-A7QbMiYHgCw&usg=AFQjCNGLCEClTQju7-2E1l7tIqkiGwuI5Q&sig2=6BsVeTjcfIjuzfpWZx5_yQ&bvm=bv.69137298,d.ZGU
  • Received 21 June 2013; Accepted 9 July 2013

Histone Deacetylases and their Inhibitors as Potential Therapeutic Drugs for Cholangiocarcinoma – Cell Line Findings

 

 

 

 

GStorm GS1

 

The Association Between HLA Class II Alleles and the Occurrence of Factor VIII Inhibitor in Thai Patients with Hemophilia A

Objective:This study aimed to investigate the association between HlA class II alleles and the occurrence of FVIII inhibitor in Thai hemophilia A patients.Material and Methods: The distribution of HlA-DRb1 alleles and DQb1 alleles in 57 Thai hemophilia A patients and 36 blood donors as controls was determined using the PCR sequence-specific primer (PCR-SSP) method, and the association between the occurrence of factor VIII (FVIII) inhibitor and the presence of certain HlA class II alleles was investigated. Results:The frequency of HlA-DRb1*15 was higher in the hemophilia A patients with and without FVIII inhibitor, whereas that of DRb1*14, DRb1*07, and DQb1*02 was lower in the hemophilia A patients with FVIII inhibitor, as compared to controls. Interestingly, only the frequency of DRb1*15 was significantly higher in the patients with inhibitor than in the controls (P = 0.021). Moreover, the frequency of DRb1*15 in the patients with inhibitor was higher than in those without inhibitor (P = 0.198).Conclusion: The study’s findings show that the DRb1*15 allele might have contributed to the occurrence of inhibitor in the Thai hemophilia A patients; however, additional research using larger samples and high-resolution DRb1 typing is warranted.

Key Words:HlA class II alleles, FVIII inhibitor, Hemophilia A, Thais


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The Association Between HLA Class II Alleles and the Occurrence of Factor VIII Inhibitor in Thai Patients with Hemophilia A
Occurrence of Baylisascaris transfugain wild populations of European brown bears (Ursus arctos) as identified by a new PCR method
Identification of a Major Genetic Determinant of Glycaemic Index in Rice
Bacterial diversity of weathered terrestrial Icelandic volcanic glasses
Embryonic gene activation in in vitro produced embryos of the domestic cat (Felis catus)
Mapping quantitative trait loci associated with yield and yield components under reproductive stage salinity stress in rice ( Oryza sativa L.)
Molecular epidemiology of Pasteurella multocida in dairy and beef calves
High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinised using mineral oil
Diversity of Global Rice Markets and the Science Required for Consumer-Targeted Rice Breeding
Characterisation of sunflower-21(SF21) genes expressed in pollen and pistil of Senecio squalidus
(Asteraceae) and their relationship with other members of the SF21gene family.
Influence of Culture Medium Composition on Relative mRNA Abundances in Domestic Cat Embryos
Human neutrophil alloantigen genotype frequencies in Thai blood donors
Frequency of FCGR3B Alleles in Thai Blood Donors
RESEARCH OF BIRD FLU VIRUS BY G-STORM GS1 PCR
Dystrophin is a tumor suppressor in human cancers with myogenic programs
Temporal variation outweighs effects of biosolids applications in shaping arbuscular mycorrhizal fungi communities on plants grown in pasture and arable soils
Probing Oral Microbial Functionality – Expression of spxB in Plaque Samples
Environmental Influences on Competitive Hydrogen Peroxide Production in Streptococcus gordonii
Contrasting Responses to Nutrient Enrichment of Prokaryotic Communities Collected from Deep Sea Sites in the Southern Ocean
Molecular Genetic Profiling of Filipino Patients with Retinoblastoma:
Investigation of age-related changes in LMNA splicing and expression of progerin in human skeletal muscles

 

 

 

Thermal Cycler Systems

 

 

 

Thermal Cycler Systems